Bruker Daltonics AutoFlex MALDI-TOF MS
An automated high-throughput MALDI TOF MS system.
The instrument uses a 337 nm N2-laser to desorb and ionise molecules. The high resolution magnifying observation optics displays the target on the PC screen and enables exact positioning of the laser. Positive or negative ions are extracted into the 122-cm linear or 260 cm reflectron flight path using the delayed extraction mode. An accessory for post-source decay analysis is also available. In addition, the Autoflex™ uses targets in a microtitre plate format that may be interfaced to a robotic sample preparation line.
Instrument Mass Range and Sensitivity
MALDI TOF is particularly suitable for the analysis of high molecular weight compounds. including the determination of molecular masses of biopolymers such as peptides and proteins, carbohydrates, oligonucleotides and synthetic polymers with relative masses up to several hundred kilodalton. The upper mass limit though is not known, but it is probably not so much determined by the desorption/ionisation processes, but rather by ion detection efficiency. The lower mass limit of MALDI TOF MS is around 500 Da due to signals arising from molecular, fragment and adduct ions of the matrix. However, small molecular weight compounds (below 500 Da) can often be analysed without the addition of matrix in a technique called laser desorption/ionisation (LDI).
The term sensitivity in connection with MALDI is generally used to describe the smallest amount of analyte deposited on the MALDI target that can be detected. Sensitivity in this context does not refer to a concentration. However, as volumes between 0.2 and 1.5 uL are usually applied onto a MALDI target, the concentration range can be easily calculated, e.g. attomole MALDI sensitivity refers to picomolar concentration. Ions generated by MALDI can be routinely mass analysed in a TOF instrument in picomole (pmol = 10-12 mol) down to low femtomole (fmol = 10-15 mol) amount. However, attomole (amol = 10-18 mol) sensitivity has also been demonstrated using a MALDI TOF MS.
Applications of MALDI TOF MS
To date, MALDI TOF mass spectrometry has been successfully used for the analysis of a wide range of different analyte molecules. Important examples are peptides and proteins including glyco- and membrane proteins and phosphopeptides, oligosaccharides, oligonucleotides including SNP analysis, lipids, molecular aggregates with non-covalent interactions, complexes of metal ions with biomolecules, and synthetic polymers. MALDI TOF mass spectrometry is one of the most important tools for the analysis of the proteome, i.e. proteomics. In addition, it is the major tool for the analysis of the products of peptide synthesis.
We offer a 2002 High-throughput MALDI-TOF mass spectrometer acquired from Bruker - in perfect working condition. Capable of hi-res measurement when used in reflectron mode. 337 nm Nitrogen laser is designed for over 100 million shots without service; this laser's shot-count is well below 2 million. Instrument is currently in-use and can be inspected.
- FlexControl software
- 2 sample plates
Call for SPECIAL Price.
Factory installation and guaranteed eligible for service contract add: $11,000 USD.